Reliability of DNA methylation measures from dried blood spots and mononuclear cells using the HumanMethylation450k BeadArray

PA Dugue; DR English; RJ MacInnis; CH Jung; JK Bassett; LM Fitzgerald; EM Wong; JE Joo; JL Hopper; MC Southey; GG Giles; RL Milne

Journal article

Reliability of DNA methylation measures from dried blood spots and mononuclear cells using the HumanMethylation450k BeadArray

PA Dugue, DR English, RJ MacInnis, CH Jung, JK Bassett, LM Fitzgerald, EM Wong, JE Joo, JL Hopper, MC Southey, GG Giles, RL Milne

Scientific Reports | NATURE PORTFOLIO | Published : 2016

DOI: 10.1038/srep30317

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Abstract

The reliability of methylation measures from the widely used HumanMethylation450 (HM450K) microarray has not been assessed for DNA from dried blood spots (DBS) or peripheral blood mononuclear cells (PBMC), nor for combined data from different studies. Repeated HM450K methylation measures in DNA from DBS and PBMC samples were available from participants in six casecontrol studies nested within the Melbourne Collaborative Cohort Study. Reliability was assessed for individual CpGs by calculating the intraclass correlation coefficient (ICC) based on technical replicates (samples repeated in a single study; 126 PBMC, 136 DBS) and study duplicates (samples repeated across studies; 280 PBMC, 769 DB..

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University of Melbourne Researchers

Pierre-Antoine Dugue Author

Dallas English Author

Robert MacInnis Author

Chol-Hee Jung Author

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Awarded by National Science Foundation

Funding Acknowledgements

We are grateful to the participants in the MCCS for participating in the study and providing blood samples. We thank Yurii Shvetsov for sending us the list of ICCs generated in their study<SUP>16</SUP>. This study was made possible thanks to the funding obtained from the National Health and Medical Research Council, including project grant number 1011618, 1026892, 1026522, 1050198, 623206, and 1043616, and program grant number 209057 and 1074383.